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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/02/2013 |
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Data da última atualização: |
20/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, V. de; FILHO, A. N.; MELO, P. de C.; FERNANDES, G. M.; FERRAUDO, A. S.; CONDE, S. de O.; FOGAÇA JÚNIOR, F. A. |
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Afiliação: |
VIVIANE DE SOUZA, CNPC; Antonio Nader Filho, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP; Poliana de Castro Melo, UNESP - Jaboticabal, SP; Guilherme Moraes Ferraudo, UNESP - Jaboticabal, SP.; Antônio Sérgio Ferraudo, UNESP - Jaboticabal, SP; Sandra de Oliveira Conde, UNESP - Jaboticabal, SP; Flavio Augusto Fogaça Junior, UNESP - Jaboticabal, SP. |
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Título: |
Molecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012. |
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Idioma: |
Português |
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Conteúdo: |
Abstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated. MenosAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The ab... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Bulk tank; Minas Gerais; PCR; PFGE. |
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Thesagro: |
Doença animal; Mamite; Staphylococcus aureus; Vaca leiteira. |
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Thesaurus Nal: |
Brazil; Dairy cows; Mammary gland diseases; Mastitis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76787/1/API-Molecular-epidemiology.pdf.pdf
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Marc: |
LEADER 02617naa a2200349 a 4500
001 1950132
005 2015-04-20
008 2012 bl uuuu u00u1 u #d
100 1 $aSOUZA, V. de
245 $aMolecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.$h[electronic resource]
260 $c2012
520 $aAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated.
650 $aBrazil
650 $aDairy cows
650 $aMammary gland diseases
650 $aMastitis
650 $aDoença animal
650 $aMamite
650 $aStaphylococcus aureus
650 $aVaca leiteira
653 $aBrasil
653 $aBulk tank
653 $aMinas Gerais
653 $aPCR
653 $aPFGE
700 1 $aFILHO, A. N.
700 1 $aMELO, P. de C.
700 1 $aFERNANDES, G. M.
700 1 $aFERRAUDO, A. S.
700 1 $aCONDE, S. de O.
700 1 $aFOGAÇA JÚNIOR, F. A.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 43, n. 4, p. 1646-1650, out./dez. 2012.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/09/1999 |
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Data da última atualização: |
05/07/2006 |
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Autoria: |
VOLL, E.; GAZZIERO, F. S.; ADEGAS, F. S.; BRIGHENTI, A. M. |
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Título: |
Dinamica de estabelecimento e competicao de desmodio (Desmodium tortuosum (Sw.) DC.) na cultura da soja. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE SOJA, 1999, Londrina. Anais... Londrina: Embrapa Soja, 1999. |
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Páginas: |
p.400. |
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Série: |
(Embrapa Soja. Documentos, 124). |
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Idioma: |
Português |
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Conteúdo: |
O desmodio (Desmodium tortuosum (Sw.) DC.) e uma planta daninha de introducao recente nas lavouras de soja do Parana e ja assume importancia economica. O objetivo do trabalho foi determinar a periodicidade, a taxa de emergencia, a sobrevivencia e a capacidade competitiva da especie com a cultura da soja cv. Embrapa-62. O experimento foi instalado em Londrina-PR e tera duracao de cinco anos. Bancos de sementes nas densidades de 0, 60, 120, 240, e 480 sementes/ms foram estabelecidos atraves de semeadura, em maio/1997, nos sistemas convencional (Scon) e direto (Sdir) da soja, apos trigo. O delineamento foi em blocos casualizados, parcelas subdivididas, fatorial 2x5, quatro repeticoes. Em 27/08/97, o levantamento do banco de sementes mostrou uma recuperacao de 10% de sementes de desmodio. Ate 28/10/97, na fase de pre-semeadura da soja, nao ocorreram emergencias de desmodio; em pos-semeadura, as emergencias foram de 1,8% (Scon) e 3,1% (Sdir). A produtividade media da soja foi de 3505 kg/ha; as estimativas de reducao pela competicao de desmodio foram de 15,5 kg/ha por planta/m2 (Scon), e de 11,3 kg/ha (Sdir). |
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Palavras-Chave: |
Emergency; Planta daninha; Soybean. |
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Thesagro: |
Emergência; Soja. |
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Thesaurus NAL: |
weeds. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01850naa a2200253 a 4500
001 1460919
005 2006-07-05
008 1999 bl uuuu u00u1 u #d
100 1 $aVOLL, E.
245 $aDinamica de estabelecimento e competicao de desmodio (Desmodium tortuosum (Sw.) DC.) na cultura da soja.
260 $c1999
300 $ap.400.
490 $a(Embrapa Soja. Documentos, 124).
520 $aO desmodio (Desmodium tortuosum (Sw.) DC.) e uma planta daninha de introducao recente nas lavouras de soja do Parana e ja assume importancia economica. O objetivo do trabalho foi determinar a periodicidade, a taxa de emergencia, a sobrevivencia e a capacidade competitiva da especie com a cultura da soja cv. Embrapa-62. O experimento foi instalado em Londrina-PR e tera duracao de cinco anos. Bancos de sementes nas densidades de 0, 60, 120, 240, e 480 sementes/ms foram estabelecidos atraves de semeadura, em maio/1997, nos sistemas convencional (Scon) e direto (Sdir) da soja, apos trigo. O delineamento foi em blocos casualizados, parcelas subdivididas, fatorial 2x5, quatro repeticoes. Em 27/08/97, o levantamento do banco de sementes mostrou uma recuperacao de 10% de sementes de desmodio. Ate 28/10/97, na fase de pre-semeadura da soja, nao ocorreram emergencias de desmodio; em pos-semeadura, as emergencias foram de 1,8% (Scon) e 3,1% (Sdir). A produtividade media da soja foi de 3505 kg/ha; as estimativas de reducao pela competicao de desmodio foram de 15,5 kg/ha por planta/m2 (Scon), e de 11,3 kg/ha (Sdir).
650 $aweeds
650 $aEmergência
650 $aSoja
653 $aEmergency
653 $aPlanta daninha
653 $aSoybean
700 1 $aGAZZIERO, F. S.
700 1 $aADEGAS, F. S.
700 1 $aBRIGHENTI, A. M.
773 $tIn: CONGRESSO BRASILEIRO DE SOJA, 1999, Londrina. Anais... Londrina: Embrapa Soja, 1999.
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Embrapa Soja (CNPSO) |
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